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NFkB p65 (phospho Ser529) antibody

Anti-NFkB p65 (phospho Ser529) antibody used in IHC (Paraffin sections) (IHC-P). GTX17575
Anti-NFkB p65 (phospho Ser529) antibody used in IHC (Paraffin sections) (IHC-P). GTX17575
Anti-NFkB p65 (phospho Ser529) antibody used in Western Blot (WB). GTX17575

Cat. No. GTX17575

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
50 μl ($539)
See all NFkB p65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human NFkB that contains serine 529.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RELA proto-oncogene, NF-kB subunit , CMCU , NFKB3 , p65

Cellular Localization

Nucleus,Cytoplasm

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-NFkB p65 (phospho Ser529) antibody used in IHC (Paraffin sections) (IHC-P). GTX17575

GTX17575 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX17575 NFkB p65 (phospho Ser529) antibody.
Right : Primary antibody
Left : a negative control without primary antibody
Dilution : 1:50 overnight at 4ºC
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 mins

Anti-NFkB p65 (phospho Ser529) antibody used in IHC (Paraffin sections) (IHC-P). GTX17575

GTX17575 IHC-P Image

IHC-P analysis of human spleen tissue using GTX17575 NFkB p65 (phospho Ser529) antibody.
Right : Primary antibody
Left : a negative control without primary antibody
Dilution : 1:20 overnight at 4ºC
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 mins

Anti-NFkB p65 (phospho Ser529) antibody used in Western Blot (WB). GTX17575

GTX17575 WB Image

WB analysis of Jurkat cells unstimulated (lane 1) or stimulated with PMA and Ca2+ (lane 2-5) using GTX17575 NFkB p65 (phospho Ser529) antibody.
Lane 1 and 2 : no peptide
Lane 3 : non-phospho peptide
Lane 4 : generic [pS] peptide
Lane 5 : phosphopeptide immunogen

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 539