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NRBP1 antibody [N1C1]

Anti-NRBP1 antibody [N1C1] used in Western Blot (WB). GTX115907
Anti-NRBP1 antibody [N1C1] used in Western Blot (WB). GTX115907
Anti-NRBP1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115907
Anti-NRBP1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX115907

Cat. No. GTX115907

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all NRBP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

Jurkat , Mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NRBP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624962

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor binding protein 1 , BCON3 , MADM , MUDPNP , NRBP

Cellular Localization

Cytoplasm , cell cortex , Endomembrane system , Cell projection , lamellipodium

Background

May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication.

Database

Research Area

DATA IMAGES

Anti-NRBP1 antibody [N1C1] used in Western Blot (WB). GTX115907

GTX115907 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
7.5% SDS PAGE
GTX115907 diluted at 1:1000

Anti-NRBP1 antibody [N1C1] used in Western Blot (WB). GTX115907

GTX115907 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX115907 diluted at 1:10000

Anti-NRBP1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115907

GTX115907 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using NRBP(GTX115907) antibody at 1:500 dilution.

Anti-NRBP1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX115907

GTX115907 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using NRBP(GTX115907) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169