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NSE antibody [N1C1]

Orthogonal
Anti-NSE antibody [N1C1] used in IHC (Frozen sections) (IHC-Fr). GTX101553
Anti-NSE antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101553
Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553
Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553
Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553
Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553
Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553

Cat. No. GTX101553

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all NSE products
PRODUCT

Summary

NSE is a 78 kDa gamma-homodimer expressed as a dominant enolase-isoenzyme in neuronal and neuroendocrine tissues. Elevated levels of serum NSE are often associated with neuronal disorders and neural crest-derived tumors. Up to 70 percent of patients with small cell lung carcinoma (SCLC) have high levels of serum NSE, as SCLC cells can express neuronal gene programs.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Mouse brain

Product Note

This antibody was raised against human NSE (ENO2) protein, but it may cross react other family members based on sequence homology.

Predict Reactivity

Zebrafish, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NSE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240765

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enolase 2 , HEL-S-279 , NSE

Cellular Localization

Cytoplasm , Cell membrane

Background

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-NSE antibody [N1C1] used in IHC (Frozen sections) (IHC-Fr). GTX101553

GTX101553 IHC-Fr Image

NSE antibody [N1C1] detects NSE protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: NSE protein stained by NSE antibody [N1C1] (GTX101553) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-NSE antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101553

GTX101553 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ENO2(GTX101553) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553

GTX101553 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody [N1C1] (GTX101553) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553

GTX101553 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX101553 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553

GTX101553 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody [N1C1] (GTX101553) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553

GTX101553 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016.PMID: 27034167

Anti-NSE antibody [N1C1] used in Western Blot (WB). GTX101553

GTX101553 WB Image

The data was published in the journal Oncotarget in 2016.PMID: 27034167

REFERENCE

Application Reference

Ugun-Klusek A et al. Cell Death Dis 2017; 8 (1) : e2531 Continued 26S proteasome dysfunction in mouse brain cortical neurons impairs autophagy and the Keap1-Nrf2 oxidative defence pathway.
Application : WB
Reactivity : Mouse
Lin TP et al. Oncotarget 2016; (Epub) REST reduction is essential for hypoxia-induced neuroendocrine differentiation of prostate cancer cells by activating autophagy signaling.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169