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NUCB2 antibody

Anti-NUCB2 antibody used in Western Blot (WB). GTX130943
Anti-NUCB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130943
Anti-NUCB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX130943
Anti-NUCB2 antibody used in Immunoprecipitation (IP). GTX130943

Cat. No. GTX130943

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all NUCB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human NUCB2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886388

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nucleobindin 2 , HEL-S-109 , NEFA

Cellular Localization

Membrane; Peripheral membrane protein , Cytoplasm , Secreted

Background

Nucleobindin-2 is a calcium-binding EF-hand protein.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-NUCB2 antibody used in Western Blot (WB). GTX130943

GTX130943 WB Image

NUCB2 antibody detects NUCB2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NUCB2 antibody (GTX130943) diluted at 1:1000.

Anti-NUCB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130943

GTX130943 ICC/IF Image

NUCB2 antibody detects NUCB2 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NUCB2 protein stained by NUCB2 antibody (GTX130943) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-NUCB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX130943

GTX130943 IHC-P Image

NUCB2 antibody detects NUCB2 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
NUCB2 antibody (GTX130943) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NUCB2 antibody used in Immunoprecipitation (IP). GTX130943

GTX130943 IP Image

Immunoprecipitation of NUCB2 protein from 293T whole cell extracts using 5 μg of NUCB2 antibody (GTX130943).
Western blot analysis was performed using NUCB2 antibody (GTX130943) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169