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Osteocalcin antibody

Anti-Osteocalcin antibody used in Western Blot (WB). GTX64348
Anti-Osteocalcin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64348
Anti-Osteocalcin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64348
Anti-Osteocalcin antibody used in Western Blot (WB). GTX64348

Cat. No. GTX64348

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Rat
Package
100 μl ($399)
See all Osteocalcin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BGLAP (NP_954642.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

bone gamma-carboxyglutamate protein , BGP , OC , OCN

Cellular Localization

Secreted

Background

This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]

Database

Research Area

DATA IMAGES

Anti-Osteocalcin antibody used in Western Blot (WB). GTX64348

GTX64348 WB Image

WB analysis of rat lung tissue lysate using GTX64348 Osteocalcin antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Osteocalcin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64348

GTX64348 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64348 Osteocalcin antibody.
Blue : DAPI
Dilution : 1:100

Anti-Osteocalcin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64348

GTX64348 ICC/IF Image

ICC/IF analysis of C6 cells using GTX64348 Osteocalcin antibody.
Blue : DAPI
Dilution : 1:100

Anti-Osteocalcin antibody used in Western Blot (WB). GTX64348

GTX64348 WB Image

WB analysis of U-87MG cell lysate using GTX64348 Osteocalcin antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Wang S et al. Front Bioeng Biotechnol 2023; 11 : 1098172 Extracellular vesicles secreted by human gingival mesenchymal stem cells promote bone regeneration in rat femoral bone defects.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399