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PDI antibody [RL77]

Anti-PDI antibody [RL77] used in IHC (Paraffin sections) (IHC-P). GTX25484
Anti-PDI antibody [RL77] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25484
Anti-PDI antibody [RL77] used in IHC (Paraffin sections) (IHC-P). GTX25484
Anti-PDI antibody [RL77] used in IHC (Paraffin sections) (IHC-P). GTX25484
Anti-PDI antibody [RL77] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25484
Anti-PDI antibody [RL77] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25484
Anti-PDI antibody [RL77] used in Flow cytometry (FACS). GTX25484
Anti-PDI antibody [RL77] used in Western Blot (WB). GTX25484
Anti-PDI antibody [RL77] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25484
Anti-PDI antibody [RL77] used in Flow cytometry (FACS). GTX25484
Anti-PDI antibody [RL77] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25484
Anti-PDI antibody [RL77] used in Flow cytometry (FACS). GTX25484

Cat. No. GTX25484

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 RL77

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS, IP, IHC, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Rat, Dog, Hamster, Pig, Golden Syrian Hamster, Xenopus, Primate
Package
100 μl ($399)
See all PDI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:2000
ICC/IF 1:100-1:1000
IHC-P 1:100
FACS Assay dependent
IP Assay dependent
IHC Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites diluted with PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Purified rat PDI protein.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prolyl 4-hydroxylase subunit beta , PDI , PDIR

Cellular Localization

Endoplasmic reticulum,Cell membrane

Background

may be identical to protein disulfide isomerase, iodothyronine 5 monodeiodinase (5MD), and membrane associated thyroid hormone binding protein MTHBP. [RGD, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-PDI antibody [RL77] used in IHC (Paraffin sections) (IHC-P). GTX25484

GTX25484 IHC-P Image

IHC-P analysis of human liver tissue using GTX25484 PDI antibody [RL77].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-PDI antibody [RL77] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25484

GTX25484 ICC/IF Image

ICC/IF analysis of A549 cells using GTX25484 PDI antibody [RL77].
Green : Primary antibody
Blue : cell nucleus

Anti-PDI antibody [RL77] used in IHC (Paraffin sections) (IHC-P). GTX25484

GTX25484 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX25484 PDI antibody [RL77].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-PDI antibody [RL77] used in IHC (Paraffin sections) (IHC-P). GTX25484

GTX25484 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX25484 PDI antibody [RL77].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-PDI antibody [RL77] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25484

GTX25484 ICC/IF Image

ICC/IF analysis of NS-1 Cells using GTX25484 PDI antibody [RL77].
Green : Primary antibody
Blue : cell nucleus

Anti-PDI antibody [RL77] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25484

GTX25484 ICC/IF Image

ICC/IF analysis of HMVEC Cells using GTX25484 PDI antibody [RL77].
Green : Primary antibody
Blue : cell nucleus

Anti-PDI antibody [RL77] used in Flow cytometry (FACS). GTX25484

GTX25484 FACS Image

FACS analysis of Hela cells using GTX25484 PDI antibody [RL77] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

Anti-PDI antibody [RL77] used in Western Blot (WB). GTX25484

GTX25484 WB Image

WB analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates using GTX25484 PDI antibody [RL77].
Dilution : 1:1000

Anti-PDI antibody [RL77] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25484

GTX25484 ICC/IF Image

ICC/IF analysis of MDCK cells using GTX25484 PDI antibody [RL77].
Green : Primary antibody
Blue : cell nucleus
Permeabilization : 0.1% Triton X-100
Dilution : 1:100 in blocking buffer for at least 1 hour at room temperature

Anti-PDI antibody [RL77] used in Flow cytometry (FACS). GTX25484

GTX25484 FACS Image

FACS analysis of 3T3 cells using GTX25484 PDI antibody [RL77] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

Anti-PDI antibody [RL77] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25484

GTX25484 ICC/IF Image

ICC/IF analysis of rat epithelial cells using GTX25484 PDI antibody [RL77].

Anti-PDI antibody [RL77] used in Flow cytometry (FACS). GTX25484

GTX25484 FACS Image

FACS analysis of K562 cells using GTX25484 PDI antibody [RL77] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

REFERENCE

Application Reference

Shen TJ et al. JCI Insight 2022; 7 (21) Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein-mediated viral translation.
Application : WB
Reactivity : Golden Syrian Hamster
Bauer CS et al. Acta Neuropathol 2022; 144 (3) : 437-464 An interaction between synapsin and C9orf72 regulates excitatory synapses and is impaired in ALS/FTD.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399