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PIN1 antibody [HL2480]

Recombinant antibody
Anti-PIN1 antibody [HL2480] used in Western Blot (WB). GTX638829
Anti-PIN1 antibody [HL2480] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638829
Anti-PIN1 antibody [HL2480] used in IHC (Paraffin sections) (IHC-P). GTX638829
Anti-PIN1 antibody [HL2480] used in IHC (Paraffin sections) (IHC-P). GTX638829
Anti-PIN1 antibody [HL2480] used in IHC (Paraffin sections) (IHC-P). GTX638829
Anti-PIN1 antibody [HL2480] used in Western Blot (WB). GTX638829
Anti-PIN1 antibody [HL2480] used in Western Blot (WB). GTX638829

Cat. No. GTX638829

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2480

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all PIN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human PIN1.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peptidylprolyl cis/trans isomerase, NIMA-interacting 1 , DOD , UBL5

Cellular Localization

Nucleus,Nucleus speckle,Cytoplasm

Background

Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-PIN1 antibody [HL2480] used in Western Blot (WB). GTX638829

GTX638829 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PIN1 antibody [HL2480] (GTX638829) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PIN1 antibody [HL2480] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638829

GTX638829 ICC/IF Image

PIN1 antibody [HL2480] detects PIN1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: NIH-3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PIN1 stained by PIN1 antibody [HL2480] (GTX638829) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PIN1 antibody [HL2480] used in IHC (Paraffin sections) (IHC-P). GTX638829

GTX638829 IHC-P Image

PIN1 antibody [HL2480] detects PIN1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PIN1 stained by PIN1 antibody [HL2480] (GTX638829) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PIN1 antibody [HL2480] used in IHC (Paraffin sections) (IHC-P). GTX638829

GTX638829 IHC-P Image

PIN1 antibody [HL2480] detects PIN1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
PIN1 stained by PIN1 antibody [HL2480] (GTX638829) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PIN1 antibody [HL2480] used in IHC (Paraffin sections) (IHC-P). GTX638829

GTX638829 IHC-P Image

PIN1 antibody [HL2480] detects PIN1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
PIN1 stained by PIN1 antibody [HL2480] (GTX638829) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PIN1 antibody [HL2480] used in Western Blot (WB). GTX638829

GTX638829 WB Image

Whole zebrafish extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with PIN1 antibody [HL2480] (GTX638829) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PIN1 antibody [HL2480] used in Western Blot (WB). GTX638829

GTX638829 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PIN1 antibody [HL2480] (GTX638829) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations