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PKC mu (phospho Ser742) antibody

Orthogonal
Anti-PKC mu (phospho Ser742) antibody used in Western Blot (WB). GTX60928

Cat. No. GTX60928

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
50 μl ($399)
See all PKC mu products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKCm/PKD that contains serine 742. The sequence is conserved in mouse.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase D1 , CHDED , PKC-MU , PKCM , PKD , PRKCM

Cellular Localization

Cytoplasm,Cell membrane

Background

The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion. [provided by RefSeq, Jan 2017]

Database

Research Area

DATA IMAGES

Anti-PKC mu (phospho Ser742) antibody used in Western Blot (WB). GTX60928

GTX60928 WB Image

Orthogonal

WB (peptide competition) analysis of K562 cells stimulated with PMA (2-6) using GTX60928 PKC mu (phospho Ser742) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 6) eliminates the signal further verifying that the antibody is phospho-specific.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399