APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:1000 |
| 1:50-1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide, corresponding to amino acids 480-530 of Human c-PLA2.
Purification
Purified by antigen-affinity chromatography
From serum
Purity
> 95% (by SDS-PAGE)
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
phospholipase A2 group IVA , PLA2G4 , cPLA2 , cPLA2-alpha
Cellular Localization
Cytoplasm,Cytoplasmic vesicle
Background
This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
Database
Research Area
DATA IMAGES
|
GTX66631 WB Image
WB analysis of various samples using GTX66631 PLA2G4A antibody.
Lane1 : A549 whole cell lysate(40μg)
Lane2 : BV2 whole cell lysate(15μg)
Dilution : 1:500
|
|
|
GTX66631 WB Image
WB analysis of various samples using GTX66631 PLA2G4A antibody.
Lane1 : THP-1 whole cell lysate(40ug)
Lane2 : MEF whole cell lysate(10ug)
Lane3 : H9C2 whole cell lysate(40ug)
Lane4 : K562 whole cell lysate(10ug)
Lane5 : Jurkat whole cell lysate(40ug)
Dilution : 1:500
|
|
|
GTX66631 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX66631 PLA2G4A antibody.
Dilution : 1:100
|
REFERENCE
There are currently no references for PLA2G4A antibody (GTX66631). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PLA2G4A antibody (GTX66631). Be the first to share your experience with this product.
