APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:100-1:1000 |
1:100-1:500 |
3-5 μg/10⁶ cells |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Synthetic phosphopeptide corresponding to residues I(8) C P L (pS) P L E A D D L(19) of mouse PPAR alpha.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
RRID
AB_372524
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
peroxisome proliferator activated receptor alpha , 4933429D07Rik , AW742785 , Nr1c1 , PPAR-alpha , PPARalpha , Ppar
Cellular Localization
Nucleus
Background
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex. The corresponding gene for the phospho-PPAR alpha is NR1C1.
Database
Research Area
DATA IMAGES
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GTX23484 WB Image
WB analysis of whole cell extracts (20 μg lysate) of U-87 MG (Lane 1), MCF7 (Lane 2), MDA-MB-231 (Lane 3), C2C12 (lane 4), Hep G2 (lane 5) and NIH/3T3 (lane 6) using GTX23484 PPAR alpha (phospho Ser12) antibody. Dilution : 1:250-1:1000
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GTX23484 ICC/IF Image
ICC/IF analysis of C2C12 cells using GTX23484 PPAR alpha (phospho Ser12) antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 ºC
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GTX23484 WB Image
WB analysis of 25 ug of C2C12 (lane 1), NIH-3T3 (lane 2), 3T3-L1 (lane 3) and HepG2 (lane 4) cell lysates using GTX23484 PPAR alpha (phospho Ser12) antibody. Dilution : 1:200
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GTX23484 ICC/IF Image
ICC/IF analysis of U-87 MG cells using GTX23484 PPAR alpha (phospho Ser12) antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 ºC
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GTX23484 ICC/IF Image
ICC/IF analysis of 3T3-L1 cells using GTX23484 PPAR alpha (phospho Ser12) antibody. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 ºC
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REFERENCE
REVIEW
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