APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rabbit, Sheep, Bovine, Cat, Dog, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.52 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PPAR gamma. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2909818
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
peroxisome proliferator activated receptor gamma , CIMT1 , GLM1 , NR1C3 , PPARG1 , PPARG2 , PPARgamma
Cellular Localization
Nucleus,Cytoplasm
Background
This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX113344 IHC-P Image
PPAR gamma antibody detects PPAR gamma protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse uterus.PPAR gamma stained by PPAR gamma antibody (GTX113344) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113344 IHC-P Image
PPAR gamma antibody detects PPAR gamma protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat uterus.PPAR gamma stained by PPAR gamma antibody (GTX113344) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113344 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPAR gamma antibody (GTX113344) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for PPAR gamma antibody (GTX113344). Be the first to share your publications with this product.
REVIEW
PPAR gamma antibody
Cat. No. GTX113344
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Rating
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( Average 4 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 18-Dec-2023
Application Tested :
WB
Sample Species :
Ms
Sample :
mouse adipose tissue
Amount used :
20μg
Blocking :
3% BSA in TBST
Primary Antibody :
1:3000, 4°C, 24Hr
