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PSMF1 antibody

Anti-PSMF1 antibody used in Western Blot (WB). GTX54613
Anti-PSMF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54613
Anti-PSMF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54613

Cat. No. GTX54613

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PSMF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-271 of human PSMF1 (NP_006805.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proteasome inhibitor subunit 1 , PI31

Cellular Localization

Cytoplasm,Endoplasmic reticulum

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PSMF1 antibody used in Western Blot (WB). GTX54613

GTX54613 WB Image

WB analysis of various sample lysates using GTX54613 PSMF1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PSMF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54613

GTX54613 IHC-P Image

IHC-P analysis of rat testis tissue using GTX54613 PSMF1 antibody.
Dilution : 1:50

Anti-PSMF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54613

GTX54613 IHC-P Image

IHC-P analysis of human liver damage tissue using GTX54613 PSMF1 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399