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Paxillin (phospho Tyr88) antibody

Anti-Paxillin (phospho Tyr88) antibody used in Western Blot (WB). GTX50350
Anti-Paxillin (phospho Tyr88) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50350

Cat. No. GTX50350

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all Paxillin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 88 (P-V-Y(p)-G-S) derived from human Paxillin.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paxillin

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-Paxillin (phospho Tyr88) antibody used in Western Blot (WB). GTX50350

GTX50350 WB Image

WB analysis of extracts from HT-29 cells untreated or treated with EGF using GTX50350 Paxillin (phospho Tyr88) antibody.

Anti-Paxillin (phospho Tyr88) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50350

GTX50350 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50350 Paxillin (phospho Tyr88) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399