APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 - 1:2000 |
1:50 - 1:100 |
1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human QARS (NP_005042.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutaminyl-tRNA synthetase , GLNRS , MSCCA , PRO2195
Cellular Localization
Cytoplasm, cytosol,Cytoplasm
Background
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. In metazoans, 9 aminoacyl-tRNA synthetases specific for glutamine (gln), glutamic acid (glu), and 7 other amino acids are associated within a multienzyme complex. Although present in eukaryotes, glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
Database
Research Area
DATA IMAGES
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GTX33449 IHC-P Image
IHC-P analysis of human liver damage tissue using GTX33449 QARS antibody. Dilution : 1:100
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GTX33449 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX33449 QARS antibody. Blue : DAPI
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GTX33449 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX33449 QARS antibody. Dilution : 1:100
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GTX33449 IHC-P Image
IHC-P analysis of human gastric cancer tissue using GTX33449 QARS antibody. Dilution : 1:100
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GTX33449 WB Image
WB analysis of various sample lysates using GTX33449 QARS antibody. Dilution : 1:1000 Loading : 25μg per lane
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REFERENCE
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REVIEW
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