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RANGAP1 antibody [1B4]

Overexpression
Anti-RANGAP1 antibody [1B4] used in IHC (Paraffin sections) (IHC-P). GTX83725
Anti-RANGAP1 antibody [1B4] used in Western Blot (WB). GTX83725
Anti-RANGAP1 antibody [1B4] used in Western Blot (WB). GTX83725
Anti-RANGAP1 antibody [1B4] used in Western Blot (WB). GTX83725
Anti-RANGAP1 antibody [1B4] used in Flow cytometry (FACS). GTX83725
Anti-RANGAP1 antibody [1B4] used in Flow cytometry (FACS). GTX83725
Anti-RANGAP1 antibody [1B4] used in Flow cytometry (FACS). GTX83725
Anti-RANGAP1 antibody [1B4] used in IHC (Paraffin sections) (IHC-P). GTX83725
Anti-RANGAP1 antibody [1B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83725
Anti-RANGAP1 antibody [1B4] used in IHC (Paraffin sections) (IHC-P). GTX83725
Anti-RANGAP1 antibody [1B4] used in Western Blot (WB). GTX83725

Cat. No. GTX83725

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1B4

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Dog
Package
100 μl ($519)
See all RanGAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human RANGAP1 (NP_002874) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Ran GTPase activating protein 1 , Fug1 , RANGAP , SD

Cellular Localization

Cytoplasm,nucleoplasm

Background

GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state.

Database

Research Area

DATA IMAGES

Anti-RANGAP1 antibody [1B4] used in IHC (Paraffin sections) (IHC-P). GTX83725

GTX83725 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83725 RANGAP1 antibody [1B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-RANGAP1 antibody [1B4] used in Western Blot (WB). GTX83725

GTX83725 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX83725 RANGAP1 antibody [1B4].
Loading : 10 ug per lane
Dilution : 1:200

Anti-RANGAP1 antibody [1B4] used in Western Blot (WB). GTX83725

GTX83725 WB Image

Overexpression

WB analysis of HEK293T cells transfected with RANGAP1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83725 RANGAP1 antibody [1B4].
Loading : 5 ug per lane

Anti-RANGAP1 antibody [1B4] used in Western Blot (WB). GTX83725

GTX83725 WB Image

WB analysis of various cell lines using GTX83725 RANGAP1 antibody [1B4].
Loading : 35 ug per lane

Anti-RANGAP1 antibody [1B4] used in Flow cytometry (FACS). GTX83725

GTX83725 FACS Image

FACS analysis of HEK293T cells transfected with either RANGAP1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83725 RANGAP1 antibody [1B4].

Anti-RANGAP1 antibody [1B4] used in Flow cytometry (FACS). GTX83725

GTX83725 FACS Image

FACS analysis of Jurkat cells using GTX83725 RANGAP1 antibody [1B4].
Red : Primary antibody
Blue : Negative control antibody

Anti-RANGAP1 antibody [1B4] used in Flow cytometry (FACS). GTX83725

GTX83725 FACS Image

FACS analysis of HeLa cells using GTX83725 RANGAP1 antibody [1B4].
Red : Primary antibody
Blue : Negative control antibody

Anti-RANGAP1 antibody [1B4] used in IHC (Paraffin sections) (IHC-P). GTX83725

GTX83725 IHC-P Image

IHC-P analysis of human liver tissue using GTX83725 RANGAP1 antibody [1B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-RANGAP1 antibody [1B4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83725

GTX83725 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with RANGAP1 plasmid using GTX83725 RANGAP1 antibody [1B4].

Anti-RANGAP1 antibody [1B4] used in IHC (Paraffin sections) (IHC-P). GTX83725

GTX83725 IHC-P Image

IHC-P analysis of human prostate tissue using GTX83725 RANGAP1 antibody [1B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-RANGAP1 antibody [1B4] used in Western Blot (WB). GTX83725

GTX83725 WB Image

WB analysis of NIH3T3 cell lysate using GTX83725 RANGAP1 antibody [1B4].
Loading : 10 ug per lane
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519