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RFC4 antibody

Anti-RFC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33465
Anti-RFC4 antibody used in Western Blot (WB). GTX33465
Anti-RFC4 antibody used in Immunoprecipitation (IP). GTX33465
Anti-RFC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33465

Cat. No. GTX33465

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399)
See all RFC4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4 (NP_002907.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

replication factor C subunit 4 , A1 , RFC37

Cellular Localization

Nucleus

Background

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RFC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33465

GTX33465 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33465 RFC4 antibody.
Blue : DAPI

Anti-RFC4 antibody used in Western Blot (WB). GTX33465

GTX33465 WB Image

WB analysis of various sample lysates using GTX33465 RFC4 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-RFC4 antibody used in Immunoprecipitation (IP). GTX33465

GTX33465 IP Image

IP analysis of K562 cell lysate using GTX33465 RFC4 antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

Anti-RFC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33465

GTX33465 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33465 RFC4 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399