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ROCK2 (phospho Ser1366) antibody [HL1071]

Recombinant antibody Orthogonal
Anti-ROCK2 (phospho Ser1366) antibody [HL1071] used in Western Blot (WB). GTX636103
Anti-ROCK2 (phospho Ser1366) antibody [HL1071] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636103
Anti-ROCK2 (phospho Ser1366) antibody [HL1071] used in Western Blot (WB). GTX636103
Anti-ROCK2 (phospho Ser1366) antibody [HL1071] used in Western Blot (WB). GTX636103

Cat. No. GTX636103

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1071

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ROCK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser1366 of human ROCK2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909948

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho associated coiled-coil containing protein kinase 2 , ROCK-II

Cellular Localization

Cytoplasm,Cell membrane,Nucleus

Background

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ROCK2 (phospho Ser1366) antibody [HL1071] used in Western Blot (WB). GTX636103

GTX636103 WB Image

Orthogonal

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with primary antibodies diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Upper : ROCK2 (phospho Ser1366) antibody [HL1071] (GTX636103)
Lower : ROCK2 antibody (GTX102619)

Anti-ROCK2 (phospho Ser1366) antibody [HL1071] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636103

GTX636103 ICC/IF Image

ROCK2 (phospho Ser1366) antibody detects ROCK2 (phospho Ser1366) protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ROCK2 (phospho Ser1366) stained by ROCK2 (phospho Ser1366) antibody (GTX636103) diluted at 1:500.

Anti-ROCK2 (phospho Ser1366) antibody [HL1071] used in Western Blot (WB). GTX636103

GTX636103 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody [HL1071] (GTX636103) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ROCK2 (phospho Ser1366) antibody [HL1071] used in Western Blot (WB). GTX636103

GTX636103 WB Image

Orthogonal

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody [HL1071] (GTX636103) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Whyte AJ et al. J Neurosci 2021; Cell adhesion factors in the orbitofrontal cortex control cue-induced reinstatement of cocaine seeking and amygdala-dependent goal seeking.
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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations