APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT1C3 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human S100A6 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
S100 calcium binding protein A4 , CAPL , FSP1 , MTS1 , P9KA , PEL98 , S100A4 , 18A2 , 42A
Background
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX57640 ICC/IF Image
ICC/IF analysis of A549 cells using GTX57640 S100A4 antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
The satining pattern is based on the publication: PMID: 20442771
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GTX57640 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57640 S100A4 antibody. Blue: DAPI Red: Wheat germ agglutinin (WGA) conjugated fluorescein Green: Primary antibody Dilution: 1:100
The satining pattern is based on the publication: PMID: 20442771
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GTX57640 WB Image
WB analysis of various samples using GTX57640 S100A4 antibody. Lane 1 : HeLa whole cell lysate Lane 2 : A549 whole cell lysate Lane 3 : NIH-3T3 whole cell lysate Lane 4 : 3T3-L1 whole cell lysate Loading : 40 μg Dilution : 1:1000
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REFERENCE
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REVIEW
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