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SIL1 antibody [3E3]

Overexpression
Anti-SIL1 antibody [3E3] used in IHC (Paraffin sections) (IHC-P). GTX83642
Anti-SIL1 antibody [3E3] used in Western Blot (WB). GTX83642
Anti-SIL1 antibody [3E3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83642
Anti-SIL1 antibody [3E3] used in Western Blot (WB). GTX83642
Anti-SIL1 antibody [3E3] used in IHC (Paraffin sections) (IHC-P). GTX83642
Anti-SIL1 antibody [3E3] used in Flow cytometry (FACS). GTX83642
Anti-SIL1 antibody [3E3] used in IHC (Paraffin sections) (IHC-P). GTX83642
Anti-SIL1 antibody [3E3] used in IHC (Paraffin sections) (IHC-P). GTX83642
Anti-SIL1 antibody [3E3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83642

Cat. No. GTX83642

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3E3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all SIL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200
ICC/IF Assay dependent
IHC-P 1:100-500
FACS Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human SIL1 (NP_071909) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SIL1 nucleotide exchange factor , BAP , MSS , ULG5

Background

Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.

Database

Research Area

DATA IMAGES

Anti-SIL1 antibody [3E3] used in IHC (Paraffin sections) (IHC-P). GTX83642

GTX83642 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83642 SIL1 antibody [3E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-SIL1 antibody [3E3] used in Western Blot (WB). GTX83642

GTX83642 WB Image

Overexpression

WB analysis of HEK293T cells transfected with SIL1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83642 SIL1 antibody [3E3].
Loading : 5 ug per lane

Anti-SIL1 antibody [3E3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83642

GTX83642 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX83642 SIL1 antibody [3E3].

Anti-SIL1 antibody [3E3] used in Western Blot (WB). GTX83642

GTX83642 WB Image

WB analysis of various cell lines using GTX83642 SIL1 antibody [3E3].
Loading : 10 ug per lane
Dilution : 1:200

Anti-SIL1 antibody [3E3] used in IHC (Paraffin sections) (IHC-P). GTX83642

GTX83642 IHC-P Image

IHC-P analysis of liver carcinoma tissue using GTX83642 SIL1 antibody [3E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-SIL1 antibody [3E3] used in Flow cytometry (FACS). GTX83642

GTX83642 FACS Image

FACS analysis of HEK293T cells transfected with either SIL1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83642 SIL1 antibody [3E3].

Anti-SIL1 antibody [3E3] used in IHC (Paraffin sections) (IHC-P). GTX83642

GTX83642 IHC-P Image

IHC-P analysis of liver tissue using GTX83642 SIL1 antibody [3E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-SIL1 antibody [3E3] used in IHC (Paraffin sections) (IHC-P). GTX83642

GTX83642 IHC-P Image

IHC-P analysis of kidney tissue using GTX83642 SIL1 antibody [3E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-SIL1 antibody [3E3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83642

GTX83642 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with SIL1 plasmid using GTX83642 SIL1 antibody [3E3].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519