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SMAD1+5+8(9) antibody

Anti-SMAD1+5+8(9) antibody used in Western Blot (WB). GTX131100

Cat. No. GTX131100

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all SMAD1+5+8(9) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

whole zebrafish

Predict Reactivity

Human (100%)| Mouse (100%)| Rat (100%)| Zebrafish (97%)| Drosophila (93%)| Bovine (100%)| Chicken (99%)| Pig (100%)| Xenopus tropicalis (99%)| Rhesus Monkey (100%)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish SMAD1+5+8(9)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886423

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Involved in ventralization. May mediate Bmp2b signaling during embryonic dorsal-ventral pattern formation, and may itself be a transcriptional target for Smad5-mediated Bmp2b signaling.

Research Area

DATA IMAGES

Anti-SMAD1+5+8(9) antibody used in Western Blot (WB). GTX131100

GTX131100 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SMAD1+5+8(9) antibody (GTX131100) diluted at 1:500.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169