APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.3-1μg/ml |
5μg/ml |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89360-PEP).
Product Note
This antibody is not expected to cross-react with SMAD1 and SMAD5.
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-HSEPLMPHNATYPD, from the internal region of the protein sequence according to NP_005896.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SMAD family member 9 , MADH6 , MADH9 , PPH2 , SMAD8 , SMAD8/9 , SMAD8A , SMAD8B
Cellular Localization
Cytoplasm , Nucleus
Background
The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]
Database
Research Area
DATA IMAGES
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GTX89360 WB Image
WB analysis of human skin lysate using GTX89360 SMAD9 antibody, Internal. Dilution : 0.3μg/ml Loading : 35μg protein in RIPA buffer
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GTX89360 ELISA Image
ELISA analysis of SMAD9 protein using GTX89360 SMAD9 antibody, Internal. This antibody was used as a detecting antibody. Dilution : 1.5μg/ml
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GTX89360 IHC-P Image
IHC-P analysis of human kidney using GTX89360 SMAD9 antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 5μg/ml
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REFERENCE
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REVIEW
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