APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone 4E11 is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SNAP27 protein.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
synaptosome associated protein 25 , CMS18 , RIC4 , SEC9 , SNAP , SNAP25 , SUP , bA416N4.2 , dJ1068F16.2
Cellular Localization
Cytoplasm,Perinuclear region,Cell membrane,Cell junction,Synapse,Synaptosome
Background
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX57559 WB Image
WB analysis of Mouse brain tissue lysate using GTX57559 SNAP25 antibody. Loading : 30 μg Dilution : 1:2000
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GTX57559 ICC/IF Image
ICC/IF analysis of U87-MG cells using GTX57559 SNAP25 antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
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GTX57559 FACS Image
FACS analysis of U87-MG cells using GTX57559 SNAP25 antibody. Cell Number: 1 x 10⁶ cells Primary antibody: Red line Antibody amount: 2-5 μg
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GTX57559 WB Image
WB analysis of various samples using GTX57559 SNAP25 antibody. Lane 1 : 293T cell lysate Lane 2 : SNAP25 transfected 293T cell lysate Loading : 10 μg Dilution : 1:3000
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REFERENCE
There are currently no references for SNAP25 antibody [4E11] (GTX57559). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SNAP25 antibody [4E11] (GTX57559). Be the first to share your experience with this product.