APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 - 1:2000 |
1:50 - 1:200 |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 77-376 of human SMARCB1 (NP_001007469.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 , BAF47 , CSS3 , INI1 , MRD15 , PPP1R144 , RDT , RTPS1 , SNF5 , SNF5L1 , SWNTS1 , Sfh1p , Snr1 , hSNFS
Cellular Localization
Nucleus
Background
The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015]
Database
Research Area
DATA IMAGES
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GTX33509 IHC-P Image
IHC-P analysis of Human epithelioid sarcoma (ini-1 deletion) tissue using GTX33509 SNF5 antibody. Antigen retrieval : 10 mM citrate buffer pH 6.0 Dilution : 1:200
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GTX33509 IHC-P Image
IHC-P analysis of undifferentiated carcinoma of human esophagus tissue using GTX33509 SNF5 antibody. Antigen retrieval : 10 mM citrate buffer pH 6.0 Dilution : 1:200
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GTX33509 WB IP Image
IP analysis of HeLa cell lysates using GTX33509 SNF5 antibody. Antibody amount : 3 μg/ 300 μg total extraction Dilution : 1:1000
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REFERENCE
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REVIEW
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