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SOD2 antibody [1H6]

Anti-SOD2 antibody [1H6] used in IHC (Paraffin sections) (IHC-P). GTX31147
Anti-SOD2 antibody [1H6] used in Western Blot (WB). GTX31147
Anti-SOD2 antibody [1H6] used in IHC (Paraffin sections) (IHC-P). GTX31147
Anti-SOD2 antibody [1H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31147
Anti-SOD2 antibody [1H6] used in Immunoprecipitation (IP). GTX31147
Anti-SOD2 antibody [1H6] used in IHC (Paraffin sections) (IHC-P). GTX31147
Anti-SOD2 antibody [1H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31147
Anti-SOD2 antibody [1H6] used in Western Blot (WB). GTX31147
Anti-SOD2 antibody [1H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31147
Anti-SOD2 antibody [1H6] used in IHC (Paraffin sections) (IHC-P). GTX31147

Cat. No. GTX31147

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1H6

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μg ($399)
See all SOD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/mL
ICC/IF 2 μg/ml
IHC-P 1:100
IP 2 μg
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 30% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length recombinant protein of human SOD2

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

superoxide dismutase 2 , IPO-B , IPOB , MNSOD , MVCD6 , Mn-SOD

Cellular Localization

Mitochondrion matrix

Background

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. [provided by RefSeq, Apr 2016]

Database

Research Area

DATA IMAGES

Anti-SOD2 antibody [1H6] used in IHC (Paraffin sections) (IHC-P). GTX31147

GTX31147 IHC-P Image

IHC-P analysis of human kidney tissue using GTX31147 SOD2 antibody [1H6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-SOD2 antibody [1H6] used in Western Blot (WB). GTX31147

GTX31147 WB Image

WB analysis of whole cell extracts (30 μg lysate) of HepG2 (Lane 1), Jurkat (Lane 2), K562 (Lane 3), HeLa (Lane 4), U-87 MG (Lane 5), COLO 205 (Lane 6), HEK293 (Lane 7) and HUVEC (Lane 8) using GTX31147 SOD2 antibody [1H6].
Dilution : 1-2 μg/ml

Anti-SOD2 antibody [1H6] used in IHC (Paraffin sections) (IHC-P). GTX31147

GTX31147 IHC-P Image

IHC-P analysis of human cerebellum tissue using GTX31147 SOD2 antibody [1H6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-SOD2 antibody [1H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31147

GTX31147 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31147 SOD2 antibody [1H6]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton„ X-100 for 10 minutes
Dilution : 2 μg/ml in 0.1% BSA incubated for 3 hours at room temperature

Anti-SOD2 antibody [1H6] used in Immunoprecipitation (IP). GTX31147

GTX31147 IP Image

IP analysis of HepG2 cell lysates using GTX31147 SOD2 antibody [1H6].
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:500

Anti-SOD2 antibody [1H6] used in IHC (Paraffin sections) (IHC-P). GTX31147

GTX31147 IHC-P Image

IHC-P analysis of human cervical carcinoma tissue using GTX31147 SOD2 antibody [1H6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

Anti-SOD2 antibody [1H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31147

GTX31147 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31147 SOD2 antibody [1H6]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC in a humidified chamber

Anti-SOD2 antibody [1H6] used in Western Blot (WB). GTX31147

GTX31147 WB Image

WB analysis of 50 ug of various samples using GTX31147 SOD2 antibody [1H6].
Dilution : 1:500

Anti-SOD2 antibody [1H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31147

GTX31147 ICC/IF Image

ICC/IF analysis of HUVEC cells using GTX31147 SOD2 antibody [1H6]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 incubated overnight at 4 ºC in a humidified chamber

Anti-SOD2 antibody [1H6] used in IHC (Paraffin sections) (IHC-P). GTX31147

GTX31147 IHC-P Image

IHC-P analysis of human liver tissue using GTX31147 SOD2 antibody [1H6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399