APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , mouse brain , rat brain
Predict Reactivity
Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SOD2. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888223
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
superoxide dismutase 2 , IPO-B , IPOB , MNSOD , MVCD6 , Mn-SOD
Cellular Localization
Mitochondrion matrix
Background
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-SOD2 antibody [GT582] used in Western Blot (WB). GTX630558](/templates/default/js/lightbox/images/loading.gif "Anti-SOD2 antibody [GT582] used in Western Blot (WB). GTX630558")
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GTX630558 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody [GT582] (GTX630558) diluted at 1:10000.
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GTX630558 WB Image
SOD2 antibody [GT582] detects SOD2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract |
D. 30 μg HepG2 whole cell lysate/extract
12 % SDS-PAGE
SOD2 antibody [GT582] (GTX630558) dilution: 1:5000
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GTX630558 WB Image
SOD2 antibody [GT582] detects SOD2 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
SOD2 antibody [GT582] (GTX630558) dilution: 1:5000
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GTX630558 WB Image
SOD2 antibody [GT582] detects SOD2 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
12 % SDS-PAGE
SOD2 antibody [GT582] (GTX630558) dilution: 1:5000
|
REFERENCE
There are currently no references for SOD2 antibody [GT582] (GTX630558). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SOD2 antibody [GT582] (GTX630558). Be the first to share your experience with this product.
![Anti-SOD2 antibody [GT582] used in Western Blot (WB). GTX630558](/upload/website/prouct_img/small/GTX630558/GTX630558_41589_20160616_WB_shRNA_watermark_w_23061202_701.webp "Anti-SOD2 antibody [GT582] used in Western Blot (WB). GTX630558")
![Anti-SOD2 antibody [GT582] used in Western Blot (WB). GTX630558](/upload/website/prouct_img/small/GTX630558/GTX630558_41589_WB_w_23061202_990.webp "Anti-SOD2 antibody [GT582] used in Western Blot (WB). GTX630558")
![Anti-SOD2 antibody [GT582] used in Western Blot (WB). GTX630558](/upload/website/prouct_img/small/GTX630558/GTX630558_41589_WB_M_brain_w_23061202_652.webp "Anti-SOD2 antibody [GT582] used in Western Blot (WB). GTX630558")
![Anti-SOD2 antibody [GT582] used in Western Blot (WB). GTX630558](/upload/website/prouct_img/small/GTX630558/GTX630558_41589_WB_R_brain_w_23061202_604.webp "Anti-SOD2 antibody [GT582] used in Western Blot (WB). GTX630558")
![Anti-SOD2 antibody [GT582] used in Western Blot (WB). GTX630558](/upload/website/prouct_img/normal/GTX630558/GTX630558_41589_20160616_WB_shRNA_watermark_w_23061202_701.webp "Anti-SOD2 antibody [GT582] used in Western Blot (WB). GTX630558")
![Anti-SOD2 antibody [GT582] used in Western Blot (WB). GTX630558](/upload/website/prouct_img/normal/GTX630558/GTX630558_41589_WB_w_23061202_990.webp "Anti-SOD2 antibody [GT582] used in Western Blot (WB). GTX630558")
![Anti-SOD2 antibody [GT582] used in Western Blot (WB). GTX630558](/upload/website/prouct_img/normal/GTX630558/GTX630558_41589_WB_M_brain_w_23061202_652.webp "Anti-SOD2 antibody [GT582] used in Western Blot (WB). GTX630558")
![Anti-SOD2 antibody [GT582] used in Western Blot (WB). GTX630558](/upload/website/prouct_img/normal/GTX630558/GTX630558_41589_WB_R_brain_w_23061202_604.webp "Anti-SOD2 antibody [GT582] used in Western Blot (WB). GTX630558")