APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-2μg/ml |
| 3.75μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89929-PEP).
Product Note
This antibody is expected to recognize both reported isoforms according to NP_663786.1 and NP_061979.3.
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence SELVPEPRPKPA-C, from the N Terminus of the protein sequence according to NP_663786.1; NP_061979.3.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
septin 3 , SEP3 , bK250D10.3
Cellular Localization
Cytoplasm , Cytoplasm, cytoskeleton , Cell junction, synapse
Background
This gene belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, May 2018]
Database
Research Area
DATA IMAGES
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GTX89929 IHC-P Image
IHC-P analysis of human cortex using GTX89929 Septin 3 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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GTX89929 IHC-P Image
IHC-P analysis of human cerebellum using GTX89929 Septin 3 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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GTX89929 IHC-P Image
IHC-P analysis of human testis using GTX89929 Septin 3 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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GTX89929 WB Image
WB analysis of human brain lysate using GTX89929 Septin 3 antibody, N-term.
Dilution : 1μg/ml
Loading : 30μg protein in RIPA buffer
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REFERENCE
There are currently no references for Septin 3 antibody, N-term (GTX89929). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Septin 3 antibody, N-term (GTX89929). Be the first to share your experience with this product.
