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T7 tag antibody

Anti-T7 tag antibody used in Western Blot (WB). GTX128059

Cat. No. GTX128059

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all T7 tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
Not tested in other applications.

Positive Control

T7-tagged protein expressing 293T cells

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to T7 tag (MASMTGGQQMG).

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885695

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

T7 tag antibody

Research Area

DATA IMAGES

Anti-T7 tag antibody used in Western Blot (WB). GTX128059

GTX128059 WB Image

T7 tag antibody detects T7 tag protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of T7-tagged protein expressing 293T cells
12% SDS-PAGE
T7 tag antibody (GTX128059) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

T7 tag antibody Cat. No. GTX128059

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-T7 tag antibody used in Western Blot (WB). GTX128059
Date : Anonymous submitted on 02-Jul-2018
Score :
Application Tested : WB
Blocking : 5% skim milk in TBS, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169