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TRF1 antibody [TRF-78]

Anti-TRF1 antibody [TRF-78] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX10579
Anti-TRF1 antibody [TRF-78] used in Western Blot (WB). GTX10579
Anti-TRF1 antibody [TRF-78] used in Proximity ligation assay (PLA). GTX10579

Cat. No. GTX10579

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 TRF-78

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA, PLA

Reactivity

 Human
Package
50 μl ($399)
See all TRF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2-4 μg/ml
ICC/IF Assay dependent
ELISA Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

HeLa nuclear extract

Product Note

Monoclonal Anti-TRF1 recognizes human TRF1.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

human TRF1 protein produced in baculovirus.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

RRID

AB_371739

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

telomeric repeat binding factor 1 , PIN2 , TRBF1 , TRF , TRF1 , hTRF1-AS , t-TRF1

Cellular Localization

Nucleus

Background

TRF1 binds the double-stranded telomeric repeat sequence, TTAGGG.

Database

Research Area

DATA IMAGES

Anti-TRF1 antibody [TRF-78] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX10579

GTX10579 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX10579 TRF1 antibody [TRF-78] at 10 μg/mL(red)with DAPI(blue). Cells were fixed and permeabilized with 4% PFA followed by 0.5% Triton X-100.

Anti-TRF1 antibody [TRF-78] used in Western Blot (WB). GTX10579

GTX10579 WB Image

WB analysis of (1) HeLa Nuclear extract (2) HeLa (3) HEK-293T (4) U2OS (5) HepG2 (6) P19 (7) PC12 cell lysate using GTX10579 TRF1 antibody [TRF-78] at 4 μg/mL.

Anti-TRF1 antibody [TRF-78] used in Proximity ligation assay (PLA). GTX10579

GTX10579 PLA Image

The data was published in the Sci Rep in 2018. PMID: 30022091

REFERENCE

Application Reference

Watanabe S et al. Sci Rep 2018; 8 (1) : 10888 MDC1 methylation mediated by lysine methyltransferases EHMT1 and EHMT2 regulates active ATM accumulation flanking DNA damage sites.
Caslini C et al. Mol Cell Biol 2009; 29 (16) : 4519-26 MLL associates with telomeres and regulates telomeric repeat-containing RNA transcription.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399