Application Note
IHC-P: Use at a dilution of 1/50. Prolonged fixation in buffered formalin can destroy the epitope. IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
Product Note
There are two major classes of heat stable microtubule associated proteins (MAPS): MAP 2 (280 kD), and Tau (55-65 kD). Both classes of heat stable MAPS have a role in the regulation of microtubule polymerization in cells. The antibody reacts on immunoblots with Tau proteins and with Tau from Alzheimer paired helical filaments. The antibody does not recognize MAP 1, MAP 2, MAP 5 or tubulin. These antibodies bind both phosphorylated and non-phosphorylated Tau proteins.
We do not recommend use of this product for Mouse,Rat samples.
Form
Liquid
Buffer
20mM Tris-Borate, 150mM NaCl, dialyzed media RPMI 1640/D-MEM (FBS), BMC-6 carrier polysaccharides, carrier protein
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Antigen Species
Bovine
Immunogen
Purified bovine microtuble associated proteins (MAPS)
Purification
Tissue culture supernatant
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
microtubule associated protein tau , tau
Cellular Localization
Cytoplasm, cytosol , Cytoplasm, cytoskeleton , Cytoplasm , Cell projection, dendrite
Database
Research Area