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Thrombospondin 2 antibody

Anti-Thrombospondin 2 antibody used in Western Blot (WB). GTX11699
Anti-Thrombospondin 2 antibody used in Flow cytometry (FACS). GTX11699
Anti-Thrombospondin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX11699
Anti-Thrombospondin 2 antibody used in Western Blot (WB). GTX11699

Cat. No. GTX11699

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($449)
See all Thrombospondin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
ICC/IF 0.5-1μg/ml
IHC-P 0.5-1μg/ml
IHC-Fr 0.5-1μg/ml
FACS 1-3μg/1x10⁶cells
Not tested in other applications.

Calculated MW

129.99 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1% Na₂HPO₄, 0.45% NaCl, 2.5% BSA

Preservative

0.025% Thimerosal, 0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Thrombospondin 2(637-655aa EAAKTEKQVCEPENPCKDK), different from the mouse sequence by two amino acids.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Thrombospondin 2 antibody used in Western Blot (WB). GTX11699

GTX11699 WB Image

WB analysis of various samples using GTX11699 Thrombospondin 2 antibody.
Lane 1 : human HeLa whole cell lysates
Lane 2 : human 22RV1 whole cell lysates
Lane 3 : human U-87MG whole cell lysates
Lane 4 : human THP-1 whole cell lysates
Lane 5 : human PC-3 whole cell lysates
Lane 6 : human Caco-2 whole cell lysates
Dilution : 0.5 μg/mL
Loading : 50μg of sample under reducing conditions

Anti-Thrombospondin 2 antibody used in Flow cytometry (FACS). GTX11699

GTX11699 FACS Image

FACS analysis of U87-MG cells using GTX11699 Thrombospondin 2 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Cell only control
Antibody amount : 1μg/1x10⁶ cells for 30 min at 20ºC

Anti-Thrombospondin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX11699

GTX11699 IHC-P Image

IHC-P analysis of human placenta tissue using GTX11699 Thrombospondin 2 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

Anti-Thrombospondin 2 antibody used in Western Blot (WB). GTX11699

GTX11699 WB Image

WB analysis of various samples using GTX11699 Thrombospondin 2 antibody.
Lane 1 : rat brain tissue lysates
Lane 2 : mouse brain tissue lysates
Dilution : 0.5 μg/mL
Loading : 50μg of sample under reducing conditions

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Thimerosal.pdf
Package List Price ($)
$ 449