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Wnt3a antibody [3A6]

Anti-Wnt3a antibody [3A6] used in IHC (Paraffin sections) (IHC-P). GTX50033
Anti-Wnt3a antibody [3A6] used in Western Blot (WB). GTX50033

Cat. No. GTX50033

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3A6

Isotype

 IgG2a

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all Wnt3a products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1,000~1:2,000
Immunohistochemistry: Use at 1:50~100.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Wnt3a (19-352aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 3A

Cellular Localization

Secreted

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Wnt3a antibody [3A6] used in IHC (Paraffin sections) (IHC-P). GTX50033

GTX50033 IHC-P Image

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human Wnt3a (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Anti-Wnt3a antibody [3A6] used in Western Blot (WB). GTX50033

GTX50033 WB Image

WB analysis of various sample lysates using GTX50033 Wnt3a antibody [3A6].
Lane 1 : 293T whole cell lysate
Lane 2 : Wnt3a transfected 293T cell lysate
Dilution : 1:1000
Loading : 40μg per lane

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399