APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:100 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human YBX1 (NP_004550.2).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Y-box binding protein 1 , BP-8 , CBF-A , CSDA2 , CSDB , DBPB , EFI-A , MDR-NF1 , NSEP-1 , NSEP1 , YB-1 , YB1
Cellular Localization
Cytoplasm,Nucleus,Secreted
Background
This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
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GTX64401 WB Image
WB analysis of HepG2 cell lysate using GTX64401 YB1 antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX64401 IHC-P Image
IHC-P analysis of rat testis tissue using GTX64401 YB1 antibody.
Dilution : 1:100
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GTX64401 IHC-P Image
IHC-P analysis of mouse colone artery tissue using GTX64401 YB1 antibody.
Blue : DAPI
Dilution : 1:100
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GTX64401 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX64401 YB1 antibody.
Dilution : 1:100
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GTX64401 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX64401 YB1 antibody.
Blue : DAPI
Dilution : 1:100
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GTX64401 IHC-P Image
IHC-P analysis of mouse testis tissue using GTX64401 YB1 antibody.
Dilution : 1:100
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GTX64401 WB Image
WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX64401 YB1 antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX64401 IHC-P Image
IHC-P analysis of mouse colon tissue using GTX64401 YB1 antibody.
Blue : DAPI
Dilution : 1:100
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REFERENCE
There are currently no references for YB1 antibody (GTX64401). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for YB1 antibody (GTX64401). Be the first to share your experience with this product.
