GeneTex

  • Country / Location Selection

United States (US)

ZO-1 antibody, Internal

Anti-ZO-1 antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX88275

Cat. No. GTX88275

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF

Reactivity

 Human
Package
100 μg ($399)
See all ZO-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

195 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88275-PEP).

Product Note

This antibody is expected to recognize both reported isoforms (NP_003248.3; NP_783297.2)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-PKPTSQNQFSEHDKT, from the internal region of the protein sequence according to NP_003248.3; NP_783297.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tight junction protein 1 , ZO-1

Cellular Localization

Cell membrane , Cell junction

Background

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-ZO-1 antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX88275

GTX88275 ICC/IF Image

ICC/IF analysis of paraformaldehyde fixed A431 cells using GTX88275 ZO-1 antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control: Unimmunized goat IgG
Dilution : 10μg/ml

Green : Primary antibody
Blue : DAPI
Negative control: Unimmunized goat IgG
Dilution : 10μg/ml

REFERENCE

Application Reference

Yamasaki M et al. Biosci Microbiota Food Health 2024; 43 (1) : 13-22 Lactiplantibacillus plantarum 06CC2 upregulates intestinal ZO-1 protein and bile acid metabolism in Balb/c mice fed high-fat diet.
Submit a Reference

REVIEW

There are currently no reviews for ZO-1 antibody, Internal (GTX88275). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399