APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Monoclonal antibody B8E5 acts by functional dimerization of the receptor resulting in stabilization of the constitutive active receptor dimers and paradoxically induces a small decrease in carbachol affinity for the M2 receptor. It recognizes the pentapeptide VRTVE (aa 168-172) corresponding to the N-terminal part of the second extracellular loop of the human M2 receptor.
PROPERTIES
Form
Liquid
Buffer
Filter-sterilized PBS, 0.1% BSA
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
100 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide VRTVEDGECYIQFFSNAAVTFGTAI(C): Human second extracellular loop residues 168-192
Purification
Purified IgG2a
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CHRM2,HM2,cholinergic receptor muscarinic 2,mAChR M2,Muscarinic Acetylcholine Receptor 2
Cellular Localization
Cell membrane,Cell junction,Synapse,Postsynaptic cell membrane
Background
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
REFERENCE
There are currently no references for mAChR M2 antibody [B8E5] (GTX54482). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for mAChR M2 antibody [B8E5] (GTX54482). Be the first to share your experience with this product.