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mAChR M5 antibody

Anti-mAChR M5 antibody used in Western Blot (WB). GTX53955
Anti-mAChR M5 antibody used in IHC (Paraffin sections) (IHC-P). GTX53955
Anti-mAChR M5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53955

Cat. No. GTX53955

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all mAChR M5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 230-430 of human CHRM5 (NP_036257.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cholinergic receptor muscarinic 5 , HM5

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, stimulation of this receptor is known to increase cyclic AMP levels. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-mAChR M5 antibody used in Western Blot (WB). GTX53955

GTX53955 WB Image

WB analysis of various sample lysates using GTX53955 mAChR M5 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-mAChR M5 antibody used in IHC (Paraffin sections) (IHC-P). GTX53955

GTX53955 IHC-P Image

IHC-P analysis of rat brain tissue using GTX53955 mAChR M5 antibody.
Dilution : 1:100

Anti-mAChR M5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53955

GTX53955 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX53955 mAChR M5 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399