APPLICATION
Application Note
For WB: Use at a diltuion of 1:2, 000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). Not tested in other species. Optimal dilutions/concentrations should be determined by the researcher.
Calculated MW
Positive Control
HepG2, LNCAP, MCF7, SKOV3
PROPERTIES
Form
Liquid
Buffer
Serum diluted with PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Recombinant protein encoding the full-length p37 subunit ofhuman RFC purified from E. coli.
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_372908
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
replication factor C subunit 4 , A1 , RFC37
Cellular Localization
Nucleus
Background
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX70287 WB Image
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GTX70287 ICC/IF Image
p37 antibody detects p37 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p37 protein stained by p37 antibody (GTX70287) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX70287 WB Image
Detection of human p37 protein in HeLa whole cell lysate using GeneTex p37 polyclonal antiserum GTX70287.
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REFERENCE
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REVIEW
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