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p90 RSK1 (phospho Ser221) antibody

Orthogonal
Anti-p90 RSK1 (phospho Ser221) antibody used in Western Blot (WB). GTX10695
Anti-p90 RSK1 (phospho Ser221) antibody used in IHC (Paraffin sections) (IHC-P). GTX10695

Cat. No. GTX10695

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Chicken
Package
50 μl ($399)
See all p90 RSK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:10-1:100
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human RSK1 that contains serine 221. The sequence is conserved in mouse and rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S6 kinase A1 , HU-1 , MAPKAPK1A , RSK , RSK1 , p90Rsk

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-p90 RSK1 (phospho Ser221) antibody used in Western Blot (WB). GTX10695

GTX10695 WB Image

Orthogonal

WB (peptide competition) analysis of untreated A431 (Lane 1) or PMA-stimulated A431 cells (Lane 2~5) using GTX10695 p90 RSK1 (phospho Ser221) antibody prior incubated with the non-phosphopeptide (Lane 3), a generic [pS] peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

Anti-p90 RSK1 (phospho Ser221) antibody used in IHC (Paraffin sections) (IHC-P). GTX10695

GTX10695 IHC-P Image

IHC-P analysis of human brain tissue using GTX10695 p90 RSK1 (phospho Ser221) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retireval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399