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pan Cytokeratin antibody [HL2253]

Recombinant antibody Overexpression
Anti-pan Cytokeratin antibody [HL2253] used in IHC (Paraffin sections) (IHC-P). GTX638302
Anti-pan Cytokeratin antibody [HL2253] used in Western Blot (WB). GTX638302
Anti-pan Cytokeratin antibody [HL2253] used in Western Blot (WB). GTX638302

Cat. No. GTX638302

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2253

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all pan Cytokeratin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:51-1:1000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human Cytokeratin.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-pan Cytokeratin antibody [HL2253] used in IHC (Paraffin sections) (IHC-P). GTX638302

GTX638302 IHC-P Image

pan Cytokeratin antibody [HL2253] detects pan Cytokeratin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
pan Cytokeratin stained by pan Cytokeratin antibody [HL2253] (GTX638302) diluted at 1:51.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-pan Cytokeratin antibody [HL2253] used in Western Blot (WB). GTX638302

GTX638302 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with pan Cytokeratin antibody [HL2253] (GTX638302) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-pan Cytokeratin antibody [HL2253] used in Western Blot (WB). GTX638302

GTX638302 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with pan Cytokeratin antibody [HL2253] (GTX638302) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations