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CTP synthase antibody [N1C1]

Anti-CTP synthase antibody [N1C1] used in Western Blot (WB). GTX105265
Anti-CTP synthase antibody [N1C1] used in Western Blot (WB). GTX105265
Anti-CTP synthase antibody [N1C1] used in Western Blot (WB). GTX105265
Anti-CTP synthase antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105265
Anti-CTP synthase antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105265
Anti-CTP synthase antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105265

Cat. No. GTX105265

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Rat, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CTP synthase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036687

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CTP synthase 1 , CTPS , GATD5 , IMD24

Background

The catalytic conversion of UTP to CTP is accomplished by the enzyme cytidine-5-prime-triphosphate synthetase. The enzyme is important in the biosynthesis of phospholipids and nucleic acids, and plays a key role in cell growth, development, and tumorigenesis. The region to which the CTPS gene has been mapped is the location of breakpoints involved in several tumor types [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CTP synthase antibody [N1C1] used in Western Blot (WB). GTX105265

GTX105265 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CTP synthase antibody [N1C1] (GTX105265) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CTP synthase antibody [N1C1] used in Western Blot (WB). GTX105265

GTX105265 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CTP synthase antibody [N1C1] (GTX105265) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CTP synthase antibody [N1C1] used in Western Blot (WB). GTX105265

GTX105265 WB Image

The data was published in the journal Biol Open in 2014. PMID: 25326513

Anti-CTP synthase antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105265

GTX105265 ICC/IF Image

The data was published in the journal J Cell Sci in 2015.PMID: 26303200

Anti-CTP synthase antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105265

GTX105265 ICC/IF Image

The data was published in the journal J Cell Sci in 2015.PMID: 26303200

Anti-CTP synthase antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105265

GTX105265 ICC/IF Image

The data was published in the journal Biol Open in 2014. PMID: 25326513

REFERENCE

REVIEW

CTP synthase antibody [N1C1] Cat. No. GTX105265

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 19-Jul-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : HEK293T cells transfected with mouse CTPs-EGFP
Blocking : 1% BSA, 25°C, 0.5Hr
Fixation : 4%PFA
Permeabilization /Antigen retrieval : 0.2% Triton-X
Primary Antibody : 1:200, 25°C, 1Hr
Figure Note : Green: EGFP, Magenta: CTPs (detected with anti-rabbit-IgG Alexa647), Blue: DAPI
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169