APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.25-0.5μg/ml |
5-10μg/ml |
Note :
IHC-P
Human Pancreas shows strong staining of cytoplasm in acinar cells-.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX26034-PEP).
Product Note
This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2; NP_001157973.1).
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-AKVISVDELEYRQ, from the C Terminus of the protein sequence according to NP_062817.2; NP_001157974.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
eukaryotic translation initiation factor 4E nuclear import factor 1 , 4E-T , Clast4
Cellular Localization
Cytoplasm , Nucleus , Nucleus speckle
Background
The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
Database
DATA IMAGES
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GTX26034 IHC-P Image
IHC-P analysis of human pancreas using GTX26034 EIF4ENIF1 antibody, C-term. Antigen retrieval : citrate buffer pH 6 Dilution : 5μg/ml
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GTX26034 WB Image
WB analysis of 293 lysate using GTX26034 EIF4ENIF1 antibody, C-term. Dilution : 0.25μg/ml Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for EIF4ENIF1 antibody, C-term (GTX26034). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EIF4ENIF1 antibody, C-term (GTX26034). Be the first to share your experience with this product.