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5-Methylcytosine / 5-mC antibody

Anti-5-Methylcytosine / 5-mC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX21884

Cat. No. GTX21884

Reference 5

Host

 Sheep

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, IP, Dot, IHC

Reactivity

 Species independent
Package
250 μg ($399)
See all 5-Methylcytosine / 5-mC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF Assay dependent
FACS Assay dependent
IP 25-100 μg/ml
Dot Assay dependent
Apuri Assay dependent
IHC 10μg/ml
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

5-methyl Cytosine coupled to coupled to keyhole limpet hemocyanin (KLH)

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_372327

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-5-Methylcytosine / 5-mC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX21884

GTX21884 ICC/IF Image

ICC/IF analysis of rat neuron cells using GTX21884 5-Methylcytosine / 5-mC antibody.
Dilution : 5μg/ml

REFERENCE

Application Reference

Schneider L et al. Nucleic Acids Res 2012; 40 (12) : 5332-42 Neural stem cells exposed to BrdU lose their global DNA methylation and undergo astrocytic differentiation.
Application : FACS
Zhou FC et al. Birth Defects Res A Clin Mol Teratol 2011; 91 (8) : 703-15 Cellular DNA methylation program during neurulation and its alteration by alcohol exposure.
Application : IHC
Zhou FC et al. Alcohol Clin Exp Res 2011; 35 (4) : 735-46 Alcohol alters DNA methylation patterns and inhibits neural stem cell differentiation.
Application : ICC/IF
Singh RP et al. Cell Transplant 2009; 18 (10) : 1197-211 Cellular epigenetic modifications of neural stem cell differentiation.
Application : ICC/IF
Matthews RP et al. Development 2009; 136 (5) : 865-75 TNFalpha-dependent hepatic steatosis and liver degeneration caused by mutation of zebrafish S-adenosylhomocysteine hydrolase.
Application : Dot
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Package List Price ($)
$ 399