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ABCB5 antibody, Internal

Anti-ABCB5 antibody, Internal used in Flow cytometry (FACS). GTX88894
Anti-ABCB5 antibody, Internal used in Western Blot (WB). GTX88894

Cat. No. GTX88894

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human
Package
100 μg ($399)
See all ABCB5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
FACS 10μg/ml
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88894-PEP).

Product Note

This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QTQHRNTSKKAQ , from the internal region of the protein sequence according to NP_848654.3; NP_001157413.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP binding cassette subfamily B member 5 , ABCB5alpha , ABCB5beta , EST422562

Cellular Localization

Cell membrane

Background

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-ABCB5 antibody, Internal used in Flow cytometry (FACS). GTX88894

GTX88894 FACS Image

FACS analysis of PFA fixed HepG2 cells using GTX88894 ABCB5 antibody, Internal.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

Anti-ABCB5 antibody, Internal used in Western Blot (WB). GTX88894

GTX88894 WB Image

WB analysis of A431 cell lysate using GTX88894 ABCB5 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399