GeneTex

  • Country / Location Selection

United States (US)

ALK (phospho Tyr1604) antibody

Orthogonal
Anti-ALK (phospho Tyr1604) antibody used in Western Blot (WB). GTX16377
Anti-ALK (phospho Tyr1604) antibody used in Western Blot (WB). GTX16377
Anti-ALK (phospho Tyr1604) antibody used in IHC (Paraffin sections) (IHC-P). GTX16377
Anti-ALK (phospho Tyr1604) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16377

Cat. No. GTX16377

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Monkey
Package
100 μl ($399)
See all ALK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:500
IHC-P 1:50-1:100
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

176 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 1604 (G-H-Y(p)-E-D) derived from human ALK.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_2887533

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ALK receptor tyrosine kinase , CD246 , NBLST3

Cellular Localization

Cell membrane

Background

This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]

Database

Research Area

DATA IMAGES

Anti-ALK (phospho Tyr1604) antibody used in Western Blot (WB). GTX16377

GTX16377 WB Image

WB analysis of extracts from HepG2 cells using GTX16377 ALK (phospho Tyr1604) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-ALK (phospho Tyr1604) antibody used in Western Blot (WB). GTX16377

GTX16377 WB Image

Orthogonal

WB analysis of extracts from COS-7 cells (lane 2) using GTX16377 ALK (phospho Tyr1604) antibody.
Left : Primary antibody pre-incubated with the antigen specific peptide
Right : Primary antibody

Anti-ALK (phospho Tyr1604) antibody used in IHC (Paraffin sections) (IHC-P). GTX16377

GTX16377 IHC-P Image

IHC-P analysis of human brain tissue using GTX16377 ALK (phospho Tyr1604) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-ALK (phospho Tyr1604) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16377

GTX16377 ICC/IF Image

Orthogonal

ICC/IF analysis of HeLa cells using GTX16377 ALK (phospho Tyr1604) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

Application Reference

Wang X et al. Hepatology 2018; (Epub) Identification and Therapeutic Intervention of Co-activated ALK, FGFR2 and EphA5 Kinases in Hepatocellular Carcinoma.
Application : IHC-Fr
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for ALK (phospho Tyr1604) antibody (GTX16377). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399