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ARFGAP1 antibody

Anti-ARFGAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55515
Anti-ARFGAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55515
Anti-ARFGAP1 antibody used in Western Blot (WB). GTX55515
Anti-ARFGAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55515

Cat. No. GTX55515

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all ARFGAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ARFGAP1 (NP_001268411.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADP ribosylation factor GTPase activating protein 1 , ARF1GAP , HRIHFB2281

Cellular Localization

Cytoplasm,Golgi apparatus

Background

The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-ARFGAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55515

GTX55515 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX55515 ARFGAP1 antibody.
Dilution : 1:100

Anti-ARFGAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55515

GTX55515 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX55515 ARFGAP1 antibody.
Dilution : 1:100

Anti-ARFGAP1 antibody used in Western Blot (WB). GTX55515

GTX55515 WB Image

WB analysis of various sample lysates using GTX55515 ARFGAP1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-ARFGAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55515

GTX55515 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX55515 ARFGAP1 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399