Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.5 - 2 μg/mL |
2 μg/mL |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Mouse Small Intestine Tissue Lysate(GTX27939)
Product Note
This antibody is specific for ARMER C-Terminus
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
ARMER antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human ARMER.The immunogen is located within the last 50 amino acids of ARMER.
Purification
Purified by ion exchange chromatography
Conjugation
Unconjugated
RRID
AB_10721823
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
ADP ribosylation factor like GTPase 6 interacting protein 1 , AIP1 , ARL6IP , ARMER , SPG61
Cellular Localization
Endoplasmic reticulum membrane,Endoplasmic reticulum
Background
Apoptosis is important for normal development and tissue homeostasis. It is mediated by various caspases and ultimately results in the activation of endogenous endonucleases that degrade cellular DNA (1). Although apoptosis induced by endoplasmic reticulum (ER) stress is thought to be mediated by caspase-12 (2), other caspases such as caspase-9 are also thought to be activated following ER stress (3). Recently, ARMER, a novel integral ER-membrane protein was shown to protect cells from ER stress-induced apoptosis. Analysis of the caspase proteolytic cascade suggests that ARMER acts by inhibiting caspase-9 activity (3), although the mechanism for this remains unkown. It should be noted that ARMER is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.
Database
Research Area