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ASAP1 antibody

Anti-ASAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04589
Anti-ASAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04589
Anti-ASAP1 antibody used in Western Blot (WB). GTX04589
Anti-ASAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04589

Cat. No. GTX04589

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:3000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

125 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human ASAP1(Accession Q9ULH1), corresponding to amino acid residues K508-S558.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 , AMAP1 , CENTB4 , DDEF1 , PAG2 , PAP , ZG14P

Cellular Localization

Cytoplasm , Membrane

Background

This gene encodes an ADP-ribosylation factor (ARF) GTPase-activating protein. The GTPase-activating activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2), and is greater towards ARF1 and ARF5, and lesser for ARF6. This gene maybe involved in regulation of membrane trafficking and cytoskeleton remodeling. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-ASAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04589

GTX04589 IHC-P Image

IHC-P analysis of rat brain tissue using GTX04589 ASAP1 antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-ASAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04589

GTX04589 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX04589 ASAP1 antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-ASAP1 antibody used in Western Blot (WB). GTX04589

GTX04589 WB Image

WB analysis of various samples using GTX04589 ASAP1 antibody.
Lane 1 : Mouse brain tissue lysate blocked with antigen-specific peptides
Lane 2 : Mouse brain tissue lysate
Lane 3 : 3T3 cells with LPS treatment
Raw264.7 cells with H₂O₂ treatment

Anti-ASAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04589

GTX04589 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX04589 ASAP1 antibody.
Red : Primary antibody
Green : Beta-tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399