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CASPR antibody [HL3160]

Recombinant antibody Orthogonal
Anti-CASPR antibody [HL3160] used in Western Blot (WB). GTX640670
Anti-CASPR antibody [HL3160] used in Western Blot (WB). GTX640670
Anti-CASPR antibody [HL3160] used in Western Blot (WB). GTX640670

Cat. No. GTX640670

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL3160

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CASPR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

156 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human CASPR.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

contactin associated protein 1 , CASPR , CHN3 , CNTNAP , NRXN4 , P190

Cellular Localization

Membrane

Background

The gene product was initially identified as a 190-kD protein associated with the contactin-PTPRZ1 complex. The 1,384-amino acid protein, also designated p190 or CASPR for 'contactin-associated protein,' includes an extracellular domain with several putative protein-protein interaction domains, a putative transmembrane domain, and a 74-amino acid cytoplasmic domain. Northern blot analysis showed that the gene is transcribed predominantly in brain as a transcript of 6.2 kb, with weak expression in several other tissues tested. The architecture of its extracellular domain is similar to that of neurexins, and this protein may be the signaling subunit of contactin, enabling recruitment and activation of intracellular signaling pathways in neurons. [provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES

Anti-CASPR antibody [HL3160] used in Western Blot (WB). GTX640670

GTX640670 WB Image

Orthogonal

Boiled and unboiled rat tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CASPR antibody [HL3160] (GTX640670) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CASPR antibody [HL3160] used in Western Blot (WB). GTX640670

GTX640670 WB Image

Orthogonal

Boiled and unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CASPR antibody [HL3160] (GTX640670) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CASPR antibody [HL3160] used in Western Blot (WB). GTX640670

GTX640670 WB Image

Orthogonal

Unboiled various whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CASPR antibody [HL3160] (GTX640670) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
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Custom Conjugations