APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Product Note
This antibody was raised against mouse CD11b Extracellular domain.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Synthetic peptide encompassing a sequence within the Extracellular domain of mouse CD11b. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
integrin alpha M , CD11b/CD18 , CR3 , CR3A , Cd11b , F730045J24Rik , Ly-40 , MAC1 , Mac-1 , Mac-1a
Cellular Localization
Cell membrane
Background
Enables complement component C3b binding activity; heparan sulfate proteoglycan binding activity; and heparin binding activity. Contributes to cargo receptor activity. Involved in several processes, including central nervous system development; endocytosis; and positive regulation of neuron death. Acts upstream of or within several processes, including activated T cell proliferation; leukocyte migration; and microglia development. Located in external side of plasma membrane and nucleus. Is integral component of membrane. Part of integrin alphaM-beta2 complex. Is expressed in several structures, including central nervous system; heart; lymphatic vessel; thymus; and yolk sac. Human ortholog(s) of this gene implicated in lupus nephritis. Orthologous to human ITGAM (integrin subunit alpha M). [provided by Alliance of Genome Resources, Apr 2022]
Database
Research Area
DATA IMAGES
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GTX640541 WB Image
Boiled and unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD11b antibody [HL3095] (GTX640541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX640541 ICC/IF Image
CD11b antibody [HL3095] detects CD11b protein by immunofluorescent analysis.Sample: Raw264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD11b stained by CD11b antibody [HL3095] (GTX640541) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for CD11b antibody [HL3095] (GTX640541). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD11b antibody [HL3095] (GTX640541). Be the first to share your experience with this product.