CD11b antibody [HL3095]

Cat. No. GTX640541

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL3095
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Mouse
Package	100 μl ($399), 25 μl ($169)

See all CD11b products

Datasheet

View product citations for antibody GTX640541 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	1:100-1:1000

Not tested in other applications.

Calculated MW

127 kDa. ( Note )

Product Note

This antibody was raised against mouse CD11b Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of mouse CD11b. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin alpha M , CD11b/CD18 , CR3 , CR3A , Cd11b , F730045J24Rik , Ly-40 , MAC1 , Mac-1 , Mac-1a

Cellular Localization

Cell membrane

Background

Enables complement component C3b binding activity; heparan sulfate proteoglycan binding activity; and heparin binding activity. Contributes to cargo receptor activity. Involved in several processes, including central nervous system development; endocytosis; and positive regulation of neuron death. Acts upstream of or within several processes, including activated T cell proliferation; leukocyte migration; and microglia development. Located in external side of plasma membrane and nucleus. Is integral component of membrane. Part of integrin alphaM-beta2 complex. Is expressed in several structures, including central nervous system; heart; lymphatic vessel; thymus; and yolk sac. Human ortholog(s) of this gene implicated in lupus nephritis. Orthologous to human ITGAM (integrin subunit alpha M). [provided by Alliance of Genome Resources, Apr 2022]

Database

Gene ID: 16409 Itgam
UniProt: P05555 Itgam

Research Area

DATA IMAGES

GTX640541 WB Image

Boiled and unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD11b antibody [HL3095] (GTX640541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX640541 ICC/IF Image

CD11b antibody [HL3095] detects CD11b protein by immunofluorescent analysis.
Sample: Raw264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD11b stained by CD11b antibody [HL3095] (GTX640541) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty

Available soon.

Custom Conjugations