APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
3-6μg/ml |
Note :
IHC-P
Human Cortex shows staining of neuronal bodies.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88448-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_004058.1; NP_001035025.1).
Predict Reactivity
Mouse, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QILIENEDVLF, from the C Terminus of the protein sequence according to NP_001035025.1; NP_004058.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
chimerin 2 , ARHGAP3 , BCH , CHN2-3 , RHOGAP3
Cellular Localization
Membrane
Background
This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that contains a phorbol-ester/diacylglycerol (DAG)-type zinc finger, a Rho-GAP domain, and an SH2 domain. The encoded protein translocates from the cytosol to the Golgi apparatus membrane upon binding by diacylglycerol (DAG). Activity of this protein is important in cell proliferation and migration, and expression changes in this gene have been detected in cancers. A mutation in this gene has also been associated with schizophrenia in men. Alternative transcript splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, May 2014]
Database
Research Area
DATA IMAGES
|
GTX88448 IHC-P Image
IHC-P analysis of human cortex using GTX88448 CHN2 antibody, C-term. Antigen retrieval : citrate buffer pH 6 Dilution : 3.8μg/ml
|
REFERENCE
There are currently no references for CHN2 antibody, C-term (GTX88448). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CHN2 antibody, C-term (GTX88448). Be the first to share your experience with this product.