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CIP2A antibody [2G10]

Anti-CIP2A antibody [2G10] used in Western Blot (WB). GTX04245
Anti-CIP2A antibody [2G10] used in Flow cytometry (FACS). GTX04245
Anti-CIP2A antibody [2G10] used in IHC (Paraffin sections) (IHC-P). GTX04245
Anti-CIP2A antibody [2G10] used in Western Blot (WB). GTX04245
Anti-CIP2A antibody [2G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04245

Cat. No. GTX04245

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2G10

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat, Hamster, Monkey
Package
100 μl ($469)
See all CIP2A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:40
IHC-P 1:100-1:200
FACS 1 μg/1*106
ELISA Assay dependent
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris-Glycine, 150mM NaCl

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to a C-terminal portion of the human CIP2A protein.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell proliferation regulating inhibitor of protein phosphatase 2A , KIAA1524 , p90

Cellular Localization

Membrane , Cytoplasm

Background

Enables protein homodimerization activity. Predicted to act upstream of or within positive regulation of neural precursor cell proliferation and spermatogenesis. Located in cytosol and plasma membrane. [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-CIP2A antibody [2G10] used in Western Blot (WB). GTX04245

GTX04245 WB Image

WB analysis of NIH-3T3 cell lysates using GTX04245 CIP2A antibody [2G10].

Anti-CIP2A antibody [2G10] used in Flow cytometry (FACS). GTX04245

GTX04245 FACS Image

FACS analysis of CHO (A) and HEK293 (B) cells using GTX04245 CIP2A antibody [2G10].
Antibody amount : 1 μg/106 cells
Blue : Primary antibody
Orange : Isotype control

Anti-CIP2A antibody [2G10] used in IHC (Paraffin sections) (IHC-P). GTX04245

GTX04245 IHC-P Image

IHC-P analysis human colon cancer tissue using GTX04245 CIP2A [2G10] on a Bond Rx autostainer (Leica Biosystems).
Antigen retrieval : Heat induced antigen retrieval using 10mM sodium citrate buffer (pH 6.0), 20 minutes
Dilution : 1:200

Anti-CIP2A antibody [2G10] used in Western Blot (WB). GTX04245

GTX04245 WB Image

WB analysis of HeLa cell lysates using GTX04245 CIP2A antibody [2G10].

Anti-CIP2A antibody [2G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04245

GTX04245 ICC/IF Image

ICC/IF analysis of U2OS cells using CIP2A antibody [2G10].
Green : Primary antibody
Blue : DAPI
Red : Alpha-tubulin

REFERENCE

Clone Reference

Yu Liu et al. Cell Host Microbe. 2016; 19 (3) A Cytomegalovirus Peptide-Specific Antibody Alters Natural Killer Cell Homeostasis and Is Shared in Several Autoimmune Diseases
Application : WB, ICC/IF, ELISA
Reactivity : Human
Submit a Reference

REVIEW

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Package List Price ($)
$ 469